Résumé : Publiée dans le cadre du premier Congrès international d'histoire coloniale à Paris
Siècle(s) traité(s) : 20
Droits : Domaine public
Provenance : Ville de Pointe-à-Pitre. Réseau des bibliothèques
Permalien : http://www.manioc.org/patrimon/PAP11103
PAP11103

Éditeur(s) : Paris : Librairie Larousse
Siècle(s) traité(s) : 19
Droits : Domaine public
Provenance : Communauté d'agglomération de La Rochelle. Médiathèque Michel-Crépeau
Permalien : http://www.manioc.org/patrimon/MMC16023-2
MMC16023-2

Éditeur(s) : Oxford Univ Press
Résumé : Biological reference points are important tools for fisheries management. Reference points are not static, butmay change when a population's environment or the population itself changes. Fisheries-induced evolution is one mechanism that can alter population characteristics, leading to "shifting" reference points by modifying the underlying biological processes or by changing the perception of a fishery system. The former causes changes in "true" reference points, whereas the latter is caused by changes in the yardsticks used to quantify a system's status. Unaccounted shifts of either kind imply that reference points gradually lose their intended meaning. This can lead to increased precaution, which is safe, but potentially costly. Shifts can also occur in more perilous directions, such that actual risks are greater than anticipated. Our qualitative analysis suggests that all commonly used reference points are susceptible to shifting through fisheries-induced evolution, including the limit and "precautionary" reference points for spawning-stock biomass, B-lim and B-pa, and the target reference point for fishing mortality, F-0.1. Our findings call for increased awareness of fisheries-induced changes and highlight the value of always basing reference points on adequately updated information, to capture all changes in the biological processes that drive fish population dynamics.
Ices Journal Of Marine Science (1054-3139) (Oxford Univ Press), 2013-07 , Vol. 70 , N. 4 , P. 707-721

Droits : 2013 International Council for the Exploration of the Sea. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com
http://archimer.ifremer.fr/doc/00151/26228/24302.pdf
DOI:10.1093/icesjms/fst077
http://archimer.ifremer.fr/doc/00151/26228/

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy P - Oxford Open Option A Oxford University Press (OUP)
Résumé : International audience
The Pyrenean mountain range is a slowly deforming belt with continuous and moderate seismic activity. To quantify its deformation field, we present the velocity field estimated from a GPS survey of the Pyrenees spanning 18 yr. The PotSis and ResPyr networks, including a total of 85 GPS sites, were installed and first measured in 1992 and 1995–1997, respectively, and remeasured in 2008 and 2010. We obtain a deformation field with velocities less than 1 mm yr−1 across the range. The estimated velocities for individual stations do not differ significantly from zero with 95 per cent confidence. Even so, we estimate a maximum extensional horizontal strain rate of 2.0 ± 1.7 nanostrain per year in a N–S direction in the western part of the range. We do not interpret the vertical displacements due to their large uncertainties. In order to compare the horizontal strain rates with the seismic activity, we analyse a set of 194 focal mechanisms using three methods: (i) the ‘r’ factor relating their P and T axes, (ii) the stress tensors obtained by fault slip inversion and (iii) the strain-rate tensors. Stress and strain-rate tensors are estimated for: (i) the whole data set, (ii) the eastern and western parts of the range separately, and (iii) eight zones, which are defined based on the seismicity and the tectonic patterns of the Pyrenees. Each of these analyses reveals a lateral variation of the deformation style from compression and extension in the east to extension and strike-slip in the west of the range. Although the horizontal components of the strain-rate tensors estimated from the seismic data are slightly smaller in magnitude than those computed from the GPS velocity field, they are consistent within the 2σ uncertainties. Furthermore, the orientations of their principal axes agree with the mapped active faults.
ISSN: 0956-540X

hal-01171752
https://hal.archives-ouvertes.fr/hal-01171752
DOI : 10.1093/gji/ggv052

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy B - Oxford Open Option A Oxford University Press (OUP)
Résumé : International audience
Peridotite xenoliths exhumed by Quaternary alkaline magmatism in the Tahalgha district, southern Hoggar, represent fragments of the subcontinental lithospheric mantle beneath the boundary between the two major structural domains of the Tuareg Shield: the ‘Polycyclic Central Hoggar’ to the east and the ‘Western Hoggar’, or ‘Pharusian Belt’, to the west. Samples were collected from volcanic centres located on both sides of a major lithospheric shear zone at 4°35' separating these two domains. Although showing substantial variations in their deformation microstructures, equilibrium temperatures and modal and chemical compositions, the studied samples do not display any systematic changes of these features across the 4°35' fault. The observed variations rather record small-scale heterogeneities distributed throughout the study area and reflecting the widespread occurrence of vein conduits and metasomatized wall-rocks related to trans-lithospheric melt circulation during the Cenozoic. These features include partial annealing of pre-existing deformation microstructures, post-deformation metasomatic reactions, and trace-element enrichment, coupled with heating from 750–900°C (low-temperature lherzolites) to 900–1150°C (intermediate-T lherzolites and high-T harzburgites and wehrlites). Trace-element modelling confirms that the range of rare earth element (REE) variations observed in the Tahalgha clinopyroxenes may be accounted for by reactive porous flow involving a single stage of basaltic melt infiltration into a light REE (LREE)-depleted protolith. Whole-rock compositions record the final entrapment of disequilibrium metasomatic melts upon thermal relaxation of the veins–wall-rock system. The striking correlations between equilibrium temperatures and trace-element enrichment favor a scenario in which the high-temperature peridotites record advective heat transport along melt conduits, whereas the intermediate- and low-temperature lherzolites reflect conductive heating of the host Mechanical Boundary Layer. This indicates that the lithosphere did not reach thermal equilibrium, suggesting that the inferred heating event was transient and was rapidly erased by thermal relaxation down to the relatively low-temperature present-day geotherm. The low-T (<900°C) deformed lherzolites (porphyroclastic to equigranular) are characterized by only incipient annealing and LREE-depleted clinopyroxene compositions. They were only weakly affected by the Cenozoic events and could represent relatively well-preserved samples from rejuvenated Pan-African lithosphere. Extensive lithospheric rejuvenation occurred either regionally during the Pan-African orogeny, as a result of lithospheric delamination or thermomechanical erosion after thickening, or more locally along the meridional shear zones. The low-T Tahalgha lherzolites are comparable with lherzolites from Etang de Lherz, southern France, interpreted as lithospheric mantle rejuvenated by melt-induced refertilization during a late stage of the Variscan orogeny.
ISSN: 0022-3530

hal-01107410
https://hal.archives-ouvertes.fr/hal-01107410
DOI : 10.1093/petrology/egu056

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy P - Oxford Open Option A Oxford University Press (OUP)
Résumé : International audience
It is well known that sedimentary rocks having same porosity can have very different pore size distribution. The pore distribution determines many characteristics of the rock, among which its transport properties are often the most useful. Multifractal analysis is a powerful tool that is increasingly used to characterize the pore space. In this study, we performed multifractal analysis of pore distribution on simulated sedimentary rocks using the relaxed bidisperse ballistic deposition model (RBBDM). The RBBDM can generate a 3-D structure of sedimentary rocks of variable porosity by tuning the fraction p of particles of two different sizes. We also performed multifractal analysis on two samples of real sedimentary rock to compare with the simulation studies. One sample, an oolitic limestone is of high porosity (40 per cent) while the other is a reefal carbonate of low porosity, around 7 per cent. 2-D sections of X-ray microtomographs of the real rocks were stacked sequentially to reconstruct the real rock specimens. Both samples show multifractal character. The results from analysis of real rock agree quite well with the simulated structure of low porosity. The simulated rock of high porosity showed a weak multifractal nature though the real rock sample of similar porosity was found to be strongly multifractal. We propose a ‘structure parameter’ ξ which is a function of porosity and the generalized dimensions, and controls the transport properties of the rock.
ISSN: 0956-540X

hal-01174142
https://hal.archives-ouvertes.fr/hal-01174142
DOI : 10.1093/gji/ggu417

Éditeur(s) : Oxford Univ Press Inc
Résumé : Since the transition from terrestrial to marine environments poses strong osmoregulatory and energetic challenges, temporal and spatial fluctuations in oceanic salinity might influence salt and water balance (and hence, body condition) in marine tetrapods. We assessed the effects of salinity on three species of sea snakes studied by mark-recapture in coral-reef habitats in the Neo-Caledonian Lagoon. These three species include one fully aquatic hydrophiine (Emydocephalus annulatus), one primarily aquatic laticaudine (Laticauda laticaudata), and one frequently terrestrial laticaudine (Laticauda saintgironsi). We explored how oceanic salinity affected the snakes' body condition across various temporal and spatial scales relevant to each species' ecology, using linear mixed models and multimodel inference. Mean annual salinity exerted a consistent and negative effect on the body condition of all three snake species. The most terrestrial taxon (L. saintgironsi) was sensitive to salinity over a short temporal scale, corresponding to the duration of a typical marine foraging trip for this species. In contrast, links between oceanic salinity and body condition in the fully aquatic E. annulatus and the highly aquatic L. laticaudata were strongest at a long-term (annual) scale. The sophisticated salt-excreting systems of sea snakes allow them to exploit marine environments, but do not completely overcome the osmoregulatory challenges posed by oceanic conditions. Future studies could usefully explore such effects in other secondarily marine taxa such as seabirds, turtles, and marine mammals.
Integrative And Comparative Biology (1540-7063) (Oxford Univ Press Inc), 2012-08 , Vol. 52 , N. 2 , P. 235-244

Droits : The Author 2012. Published by Oxford University Press on behalf of the Society for Integrative and Comparative Biology. All rights reserved.
http://archimer.ifremer.fr/doc/00089/20064/20739.pdf
DOI:10.1093/icb/ics081
http://archimer.ifremer.fr/doc/00089/20064/

Éditeur(s) : Oxford Univ Press
Résumé : The interrogation of genetic markers in environmental meta-barcoding studies is currently seriously hindered by the lack of taxonomically curated reference data sets for the targeted genes. The Protist Ribosomal Reference database (PR2, http://ssurrna. org/) provides a unique access to eukaryotic small sub-unit (SSU) ribosomal RNA and DNA sequences, with curated taxonomy. The database mainly consists of nuclear-encoded protistan sequences. However, metazoans, land plants, macrosporic fungi and eukaryotic organelles (mitochondrion, plastid and others) are also included because they are useful for the analysis of hightroughput sequencing data sets. Introns and putative chimeric sequences have been also carefully checked. Taxonomic assignation of sequences consists of eight unique taxonomic fields. In total, 136 866 sequences are nuclear encoded, 45 708 (36 501 mitochondrial and 9657 chloroplastic) are from organelles, the remaining being putative chimeric sequences. The website allows the users to download sequences from the entire and partial databases (including representative sequences after clustering at a given level of similarity). Different web tools also allow searches by sequence similarity. The presence of both rRNA and rDNA sequences, taking into account introns (crucial for eukaryotic sequences), a normalized eight terms ranked-taxonomy and updates of new GenBank releases were made possible by a long-term collaboration between experts in taxonomy and computer scientists.
Nucleic Acids Research (0305-1048) (Oxford Univ Press), 2013-01 , Vol. 41 , N. D1 , P. D597-D604

Droits : The Author(s) 2012. Published by Oxford University Press.
http://archimer.ifremer.fr/doc/00114/22492/20174.pdf
DOI:10.1093/nar/gks1160
http://archimer.ifremer.fr/doc/00114/22492/

Éditeur(s) : HAL CCSD Oxford University Press
Résumé : International audience
Brain Aromatase, Estrogens, and Behavior

hal-01122072
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122072

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Previous work has demonstrated that geoelectrical measurements, acquired either along the Earth’s surface or in boreholes, can be sensitive to the presence of fractures. However, a lack of numerical approaches that are well suited to modelling electric current flow in fractured media prevents us from systematically exploring the links between geoelectrical measurements and fractured rock properties. To address this issue, we present a highly computationally efficient methodology for the numerical simulation of geoelectrical data in 2.5-D in complex fractured domains. Our approach is based upon a discrete-dual-porosity formulation, whereby the fractures and rock matrix are treated separately and coupled through the exchange of electric current between them. We first validate our methodology against standard analytical and finite-element solutions. Subsequent use of the approach to simulate geoelectrical data for a variety of different fracture configurations demonstrates the sensitivity of these data to important parameters such as the fracture density, depth, and orientation.
ISSN: 0956-540X

hal-01622151
https://hal.archives-ouvertes.fr/hal-01622151
https://hal.archives-ouvertes.fr/hal-01622151/document
https://hal.archives-ouvertes.fr/hal-01622151/file/sanzGJI2017.pdf
DOI : 10.1093/gji/ggx080

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy B - Oxford Open Option B
Résumé :  
absent
ISSN: 0829-318X

hal-01032128
https://hal.archives-ouvertes.fr/hal-01032128
DOI : 10.1093/treephys/28.10.1513

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : Understanding the genetic mechanisms of speciation and basis of species differences is among the most important challenges in evolutionary biology. Two questions of particular interest are what roles divergent selection and chromosomal differentiation play in these processes. A number of recently proposed theories argue that chromosomal rearrangements can facilitate the development and maintenance of reproductive isolation and species differences by suppressing recombination within rearranged regions. Reduced recombination permits the accumulation of alleles contributing to isolation and adaptive differentiation and protects existing differences from the homogenizing effects of introgression between incipient species. Here, we examine patterns of genetic diversity and divergence in rearranged versus collinear regions in two widespread, extensively hybridizing sunflower species, Helianthus annuus and Helianthus petiolaris, using sequence data from 77 loci distributed throughout the genomes of the two species. We find weak evidence for increased genetic divergence near chromosomal break points but not within rearranged regions overall. We find no evidence for increased rates of adaptive divergence on rearranged chromosomes; in fact, collinear chromosomes show a far greater excess of fixed amino acid differences between the two species. A comparison with a third sunflower species indicates that much of the nonsynonymous divergence between H. annuus and H. petiolaris probably occurred during or soon after their formation. Our results suggest a limited role for chromosomal rearrangements in genetic divergence, but they do document substantial adaptive divergence and provide further evidence of how species integrity and genetic identity can be maintained at many loci in the face of extensive hybridization and gene flow.
ISSN: 0737-4038

hal-01032153
https://hal.archives-ouvertes.fr/hal-01032153
DOI : 10.1093/molbev/msp043

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Several studies, both theoretical and experimental, show that sedimentary rocks have a fractal pore-grain interface. In this paper a computer simulated 3-D sedimentary rock structure generated by the Relaxed Ballistic Bidisperse Deposition Model (RBBDM), is investigated to characterize the micro structure of its pores. The pore volume and the rock-pore interface show the same fractal dimension indicating that the pore volume is a fractal. The two point density correlation is computed for the pore space and the results compare favourably with the range reported from experiments. An array of 2-D X-ray tomography micrograph sections of a real sedimentary rock, an oolitic limestone (pure calcite) from the Mondeville formation of Middle Jurassic age (Paris Basin, France), was used to generate a 3-D bitmap. The 3-D real rock sample generated in this manner, was analysed for similar studies as the simulated structure. The results were compared with those obtained from simulation. The simulation results agree qualitatively with the real rock sample. Diffusion through the connected pore space of the simulated structure was studied using a random walk algorithm and the results compared with the similar simulation study done on the 3-D oolitic limestone specimen. In both cases diffusion was found to be anomalous indicating that the sedimentary rock has a fractal geometry. The favourable comparability of results between the simulated and real rock supports the usefulness of the model of sedimentary rock generation which can be applicable to transport phenomena.
ISSN: 0956-540X

hal-00823061
https://hal.archives-ouvertes.fr/hal-00823061
DOI : 10.1093/gji/ggs084

Éditeur(s) : HAL CCSD Oxford University Press (OUP): Policy B - Oxford Open Option D
Résumé : International audience
The aim was to compare the relationship between body composition and body size in two subgroups with different metabolic risk.Body-mass index z-scores (BMIZ), bicipital, tricipital, subscapular and iliac thicknesses were determined in 178 India-originatingGuadeloupian (IOG) adolescents and 481 controls of other origins. Various equations were tested in a regression approach to fit therelationship between BMIZ and iliac thickness, and BMIZ and sum of skinfold thickness. A shift towards higher iliac thickness for a givenBMIZ was observed in IOG adolescents. This supports the idea that the relationship between BMI and risk for non-communicable diseases isethnicity-dependant.
ISSN: 1101-1262

hal-01143534
https://hal.univ-antilles.fr/hal-01143534
DOI : 10.1093/eurpub/cks070

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
We present new archaeointensity results from three Italian kilns situated at Ascoli Satriano, Vagnari and Fontanetto Po obtained with the Thellier modified by Coe double heating method. These data complement the directional results previously published. All sites are dated on the basis of archaeological information and/or thermoluminescence dating. The results are corrected for the anisotropy of the thermoremanent magnetization and the cooling rate effects. The new data are compared with previously published archaeointensity data from Italy and nearby countries within 900 km radius from Viterbo. An initial data set including archaeointesity data mainly coming from Italy, France, Switzerland, Czech Republic, Slovakia, Hungary, Greece and Bulgaria has been compiled. After the application of strict selection criteria, the most reliable data have been used for the calculation of a preliminary Italian intensity secular variation (SV) curve for the last 3000 yr. The new curve covers the 300 BC-400 AD and 1200-1900 AD periods. It is established by means of sliding windows of 200 yr shifted by 100 yr. The lack of reliable data for the 1000-200 BC and 400-1200 AD time intervals does not permit the calculation of a continuous curve. Clearly, more high-quality archaeointensity data from Italy and Europe are still needed to draw a robust intensity SV curve for the Italian Peninsula that could be used for archaeomagnetic dating in combination with the directional data.
ISSN: 0956-540X

hal-00828691
https://hal.archives-ouvertes.fr/hal-00828691
DOI : 10.1093/gji/ggs120

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : Architectural analyses of temperate tree species using a chronological approach suggest that the expression of epicormic branches is closely related to low growth rates in the axes that make up the branching system. Therefore, sole consideration of epicormic criteria may be sufficient to identify trees with low secondary growth levels or with both low primary and secondary growth levels. In a tropical tree such as Dicorynia guianensis (basralocus), where chronological studies are difficult, this relationship could be very useful as an easily accessible indicator of growth potentials. A simple method of architectural tree description was used to characterize the global structure of more than 1650 basralocus trees and to evaluate their growth level. Measurements of simple growth characters [height, basal diameter, internode length of submittal part (top of the main axis of the tree)] and the observation of four structural binary descriptors on the main stem (presence of sequential branches and young epicormic branches, state of the submittal part, global orientation), indicated that epicormic branch formation is clearly related to a decrease in length of the successive growth units of the main stem. Analysis of height vs. diameter ratios among different tree subgroups, with and without epicormic branching, suggested that trees with epicormic branches generally have a low level of secondary growth compared with primary growth.
ISSN: 0305-7364

hal-01032037
https://hal.archives-ouvertes.fr/hal-01032037
DOI : 10.1093/aob/mcg119

Éditeur(s) : Oxford Univ Press
Résumé : Spatio-seasonal explicit simulation models can predict the impact of spatial management measures on marine fish populations and fishing activities. As fisheries are complex systems, fisheries simulation models are often complex, with many uncertain parameters. Here, the methodology is provided to deliver fishery diagnostics within an uncertainty context using a complex simulation tool. A sensitivity analysis of the model is performed on model outputs using partial least-squares to identify the most sensitive parameters. The impact of several management measures is then simulated using a statistical simulation design taking into account the uncertainty of the selected sensitive parameters. This approach was applied to the Bay of Biscay anchovy stock using the ISIS-Fish (Integration of Spatial Information for Simulation of Fisheries) model to assess the impact of imposing marine protected areas (MPAs) conditionally on parameter uncertainty. The diagnostic appeared to be highly sensitive to the mortality of larvae and juveniles, growth, and reproduction. The uncertainty of the values of these parameters did not permit any of the simulated MPA designs to be proposed. However, according to anchovy catch and biomass, the simulations allowed the low impact of closure duration to be shown and underscored the utility of protecting such key processes as spawning.
Ices Journal Of Marine Science (1054-3139) (Oxford Univ Press), 2010-07 , Vol. 67 , N. 5 , P. 1063-1075

Droits : 2010 ICES/CIEM. Oxford journals
http://archimer.ifremer.fr/doc/00011/12234/9281.pdf
DOI:10.1093/icesjms/fsq002
http://archimer.ifremer.fr/doc/00011/12234/

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
Phase equilibrium data pertaining to melting of simplified carbonated peridotite in the systems CaO-MgO-SiO2-CO2 and CaO-MgO-Al2O3-SiO2-CO2 at pressures of 10-26 GPa, corresponding to similar to 300-750 km depths in the Earth, are presented. In both the studied systems, liquid compositions, with changing crystalline phase assemblage, are carbonatitic throughout the studied pressure range. In the system CMS-CO2, melting phase relations are isobarically invariant; liquid is in equilibrium with forsterite + clinoenstatite + clinopyroxene + magnesite, forsterite + majorite + clinopyroxene + magnesite, wadsleyite + majorite + clinopyroxene + magnesite, ringwoodite + majorite + calcium-silicate perovskite + magnesite, magnesium-silicate perovskite + periclase + calcium-silicate perovskite + magnesite at 12, 14, 16, 20, and 26 GPa, respectively. In the system CMAS-CO2, a phase assemblage consisting of forsterite + orthopyroxene + clinopyroxene + magnesite + garnet + melt from 10 to 14 GPa is isobarically invariant. However, owing to the disappearance of orthopyroxene at pressures greater than 14 GPa, from 16 and up to at least 26 GPa, the solidus of simplified carbonated peridotite spans a divariant surface in pressure-temperature space. The liquid coexists with wadsleyite + clinopyroxene + garnet + magnesite, ringwoodite + calcium-silicate perovskite + garnet + magnesite, and magnesium-silicate perovskite + periclase + calcium-silicate perovskite + magnesite at 16, 20, and 26 GPa, respectively. A curious, and as yet unexplained, feature of our study is an abrupt drop in the solidus temperature between 14 and 16 GPa that causes a small amount of melting of carbonated mantle in the Transition Zone of the Earth. In the systems CMS-CO2 and CMAS-CO2 liquid compositions at 16 and 20 GPa are highly calcic bona fide carbonatites; however, these liquids revert to being magnesiocarbonatites at 10-14 and 26 GPa. In the system CMS-CO2, at 16 GPa we locate an isobaric invariant point consisting of wadsleyite + clinopyroxene + anhydrous B + magnesite + melt. The presence of anhydrous B at 16 GPa and 1475 degrees C is interesting, as it lies outside the composition space of the mantle peridotite analog we have studied. However, despite the presence of two highly magnesian silicate crystalline phases, wadsleyite and anhydrous B, at 16 GPa and 1475 degrees C, the liquid composition remains calcic with molar Ca-number [Ca/(Ca + Mg) x 100] of about 63. The melting reactions at 16 and 20 GPa (with or without anhydrous B) show that lime-bearing crystalline silicates play a fairly large part in generating and controlling the composition of the liquids. At 16 GPa, in the system CMS-CO2, we also report an experimental run at 1575 degrees C, in which liquid coexists with only wadsleyite and majorite. The liquid composition is less calcic (Ca-number 54) than that for other runs at lower temperatures, but is still more calcic than liquids at 10-14 and 26 GPa in both the studied systems. At present, the likely cause for these changes in the reported phase relations is not known. For normally assumed mantle temperatures, melting in the Transition Zone of the Earth, owing to the presence of carbonate, is probably unavoidable. The depth range of the drop in the carbonated peridotite solidus closely matches that of commonly observed low seismic velocities at similar to 400-600 km depth in the Earth.
ISSN: 0022-3530

hal-00669945
https://hal.archives-ouvertes.fr/hal-00669945
DOI : 10.1093/petrology/egr048

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
BACKGROUND: Mammalian spermatogenesis is a process that involves a complex expression program in both somatic and germ cells present in the male gonad. A number of studies have attempted to define the transcriptome of male meiosis and gametogenesis in rodents and primates. Few human transcripts, however, have been associated with testicular somatic cells and germ cells at different post-natal developmental stages and little is known about their level of germline-specificity compared with non-testicular tissues. METHODS: We quantified human transcripts using GeneChips and a total of 47 biopsies from prepubertal children diagnosed with undescended testis, infertile adult patients whose spermatogenesis is arrested at consecutive stages and fertile control individuals. These results were integrated with data from enriched normal germ cells, non-testicular expression data, phenotype information, predicted regulatory DNA-binding motifs and interactome data. RESULTS: Among 3580 genes for which we found differential transcript concentrations in somatic and germ cells present in human testis, 933 were undetectable in 45 embryonic and adult non-testicular tissues, including many that were corroborated at protein level by published gene annotation data and histological high-throughput protein immunodetection assays. Using motif enrichment analyses, we identified regulatory promoter elements likely involved in germline development. Finally, we constructed a regulatory disease network for human fertility by integrating expression signals, interactome information, phenotypes and functional annotation data. CONCLUSIONS: Our results provide broad insight into the post-natal human testicular transcriptome at the level of cell populations and in a global somatic tissular context. Furthermore, they yield clues for genetic causes of male infertility and will facilitate the identification of novel cancer/testis genes as targets for cancer immunotherapies.
ISSN: 0268-1161

hal-00877243
https://hal.archives-ouvertes.fr/hal-00877243
DOI : 10.1093/humrep/des301
PUBMED : 22926843

Éditeur(s) : Paris : Hachette
Siècle(s) traité(s) : 18
Droits : Domaine public
Provenance : Ville de Pointe-à-Pitre. Réseau des bibliothèques
Permalien : http://www.manioc.org/patrimon/PAP11109
PAP11109